Figure 12

Automated Cellular Viability Analysis. The detected instances in the different stainings (Hoechst, Calcein, PI) are visualized by marking the boundaries. Thereby, different instances are encoded by changing colors. At the end of the fusion step, live cells (Hoechst positive, Calcein positive, PI negative) needed to obtain cellular viability are marked with a green cross. For reasons of visualization and clarity, the images shown represent only a crop of an entire DMA spot.