Figure 4
From: A common variant rs2054564 in ADAMTS17 is associated with susceptibility to lumbar spondylosis
Promotion of extracellular microfibril biogenesis by ADAMTS17. (a) Immunocytochemistry of MG63 cells transfected with an expression vector of ADAMTS17 or empty vector (EV) revealed the promotion of extracellular microfibril assembly by exogenous expression of ADAMTS17. Red fibers represent extracellular fibrillin fibers. Nuclei are visualized using Hoechst 33342. Upper and lower rows show representative images of different experiments. (b) qRT-PCR confirmed the robust overexpression of ADAMTS17 in MG63 cells transfected with an expression vector of ADAMTS17. (c) Quantification of the extracellular microfibrils by positive areas confirmed significantly increased microfibril biogenesis by exogenous ADAMTS17 expression. (d) Quantification of the extracellular microfibrils by total signal intensities confirmed significantly increased microfibril biogenesis by exogenous ADAMTS17 expression. For quantification in both (c,d), the four images of ADAMTS17 group and the five images of EV group were automatically quantified using a hybrid cell count application in the BZ-X Analyzer software (KEYENCE, Japan). EV empty vector.
