Table 1 Reagents used for flow cytometry validation.
From: Label-free macrophage phenotype classification using machine learning methods
Monoclonal antibody | Fluorophore | Clone | Company | Catalogue No |
|---|---|---|---|---|
CD86 | Brilliant Violet 785 | GL-1 | Biolegend | 105,043 |
CD69 | PE-Cy7 | H1.2F3 | BD Biosciences | 552,879 |
CD206 | Alexa Fluor 700 | C068C2 | Biolegend | 141,734 |
TLR1 | eFluor660 | eBioTR23 (TR23) | Thermofisher | 50,901,182 |
iNOS | PE | CXNFT | Thermofisher | 12–5920-80 |
CD204 | PE | M204PA | eBioscience | 12–2046-80 |
VEGF | PE | VG1 | Novus | NOVNB100664PE |
IL-12/IL-23 p40 | APC | C15.6 | Biolegend | 505,205 |
eBioscience™ Fixable Viability Dye | eFluor™ 780 | Thermofisher | 65–0865-14 | |
Viability Dye | DAPI | Abcam | ab228549 | |
PI | ThermoFisher | P3566 |
