Figure 4
Edema factor, lethal factor and protective antigen secretion by B. anthracis Sterne 34F2 are perturbed in the absence of anthrose. To confirm luminescent expression studies B. anthracis Sterne WT, ΔantC, and ΔantC/COMP strains were grown in HIB + Km 10 in the presence of protease inhibitors in triplicate, filter purified 24 h supernatant samples. The ODs at 600 nm were measured over time (A) and the supernatants were blotted for (B) Edema factor, (C) Lethal factor, or (D) Protective antigen. Individual band intensities, their average, and SD are shown as a percent of wildtype (WT). A repeated measures one-way ANOVA was run on the growth curve timepoints and the optical densities were not significantly different than WT in (A). One-way ANOVA tests were used to determine significant differences of the band intensities in (B–D). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
