Figure 5
Anthrose induces changes in expression of protective antigen and affects global gene regulation in B. anthracis Sterne. (A) The B. anthracis Sterne protective antigen (PA) luminescent reporter strain was grown with (purple line) and without (green line) 100 μg/ml pure anthrose for 48 h. (B) The B. anthracis Sterne ΔantC protective antigen (PA) luminescent reporter strain was grown in BHI broth with (purple line) and without (red line)100 μg/ml pure anthrose for 48 h. Growth curves were carried out in triplicate with the OD at 600 nm and the average relative luminescent units (RLU) and standard error of the mean presented at each time point. (C) Experimental design to measure global transcriptomic levels at 30 min and 2 h after adding 10 μg/ml of pure anthrose to log-phase B. anthracis Sterne grown in BHI. Each sample was collected from three experiments and submitted to RNA-seq. (D) Gene expression of B. anthracis Sterne 30 min after addition of 10 μg/ml of pure anthrose compared to mock (water) treated culture grown in parallel and (E) after 2 h compared to mock (water) treated culture grown in parallel. Red dots indicate significant genes that experience a fold-change greater than 2 or less than -2 and have a false discovery rate less than 0.05. (F) String network functional analysis of 30 min gene clusters with BAS loci labels. The red cluster are related to glucokinase processes, the teal are ATP-transmembrane processes, and yellow are involved in sugar metabolism. Lines connecting genes are different evidence of interaction. (G) String network functional analysis of 2 h gene clusters with BAS locus labels. Nucleoside monophosphate (GMP and CTP) biosynthetic processes are part of the red cluster, the salmon group is glycolytic processes, yellow are other carbon metabolic processes, and green are involved in chemotaxis/two-component systems. Lines connecting genes are different evidence of interaction.
