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Figure 1

From: Parallel recovery of chromatin accessibility and gene expression dynamics from frozen human regulatory T cells

Figure 1

The complexity of frozen samples recapitulates that of the fresh samples. Viability (a) and absolute cell count (b) of the fresh and thawed PBMC samples. (c) Recovery (%) of live PBMC recovery after thawing. The cell viability and recovery of PBMC samples were determined using trypan blue dye exclusion test. (d) Flow cytometric profile from a representative fresh and thawed PBMCs sample and the gating strategy to isolate Conventional T (Tconv) and Regulatory T (Treg) cells. (e–f) Colour dot plots showing events from fresh (e) and thawed (f) CD4 positive cells contributing to t-SNE (t-distributed stochastic neighbour embedding) distribution with manual clustering.

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