Figure 2
The quality of ATAC-seq from thawed Treg cells closely recapitulates that of the fresh cells. (a) The insert size distribution of fresh and thawed Treg ATAC-seq libraries. (b) Full library complexity and extrapolated yield curve of fresh and thawed ATAC-seq libraries. Complexity measurements are plotted against highest complexity (100% uniquely mapped reads). (c) Distribution of ATAC-seq signal at ± 1.5 kb transcriptional start sites (TSS). Signal coverage is calculated from reads per million mapped reads for each sample. (d) Percentage of reads mapping to mitochondrial genome. Deeper colour is used to depict the most desirable value of the statistic and range following a linear scale starting at 0 (black) and ending at the maximum value (yellow). All values were determined from the full depth of aligned reads. Statistical significance of the difference between the fresh and thawed samples is computed by Paired Student’s t-Test. The data shown in a-c are representative of sequencing reads pooled from three donors.
