Figure 2 | Scientific Reports

Figure 2

From: Transport of Designed Ankyrin Repeat Proteins through reconstituted human bronchial epithelia and protection against SARS-CoV-2

Figure 2

Confocal Laser Scanning Microscopy (CLSM) of reconstituted HBE incubated with fluorescence-labeled DARPins or control antibody. Multivalent DARPin ALE058 (A-D), monovalent HSA-binding DARPin domain MWA010 (E–H), negative control DARPin PSC078 (I–L) and REGN10933 antibody as positive control (M–P) were added to the basal cell culture medium together with the nuclear dye Hoechst 33342 and lipophilic membrane dye CellBrite® Red. Cell cultures were fixed at 24 h with 4% formaldehyde for later imaging. Columns from left to right show nuclei in blue, Atto-488 labeled candidate and control molecules in green, membranes in magenta, and the overlays. The superposition of the green fluorescent signal of the Atto-488 labeled molecules with the red signal of the membrane dye results in white in the composite images (arrow heads). Scale bars: 25 μm.

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