Figure 3
Antiviral activity of ALE058, REGN10933 and Remdesivir in HBE infected with SARS-CoV-2. HBE cultures were treated with either (i) ALE058 or REGN10933 at 10, 40 or 160 µg/mL, (ii) Remdesivir at 5 µM, or (iii) mock treated from the basolateral side, 2 days before infection with 104 TCID50 SARS-CoV-2 from the apical side. The protective capacity of the compounds was assessed by (A) determining the infectious virus titer (TCID50/mL) in apical washes with a limiting dilution assay at 96 hpi, with the limit of detection (LOD) at 10 infectious particles/mL, and (B) measuring the viral genome equivalents/mL in apical washes by qRT-PCR at 1, 72 and 96 hpi. Results are shown as boxplots of 6 individual cell cultures (all orginating from the same donor lung) from 2 separate experiments. Boxes display interquartile ranges with the central bar indicating the median and whiskers minimal and maximal values. ****p < 0.0001; n.s. not statistically significant, LOD limit of detection.
