Figure 5

RT-qPCR validation of the differentially expressed miRNAs. (A Expression levels of six differentially expressed miRNAs normalized to the expression level of U6 of E. sinensis with different treatments.) (B Expression levels of four target CLOCK system genes normalized to the expression level of β-actin of E. sinensis with different treatments.) Groups marked "*" represented a significant difference at P < 0.05, "**" represented a significant difference at P < 0.01, "***" represented a significant difference at P < 0.001. Statistical analyses were performed using a one-way ANOVA test in GraphPad Prism (V 8.0).