Table 1 Experimental conditions and settings: oxygen concentration, cg and ci, in the supplied gas mixtures to the gas channels and the stage incubator, respectively, and time period in validation experiment or cellular experiment.

From: Microfluidic platform for the reproduction of hypoxic vascular microenvironments

Condition

O2 concentration [%]

Time period [h]

Gas channels

cg

Stage incubator

ci

Validation expt

Cellular expt

Nstat

21

21

2

6

H10stat

10

21

–

–

H5stat

5

21

–

–

H3stat

3

21

5

6

H1stat

1

21

–

–

H0stat

0

21

5

–

Nflow

21

21

–

6

H3flow

3

3

3

6

H0flow

0

0

8

–

R0–R10

0–10

0–10

≥ 2

–

  1. The normoxic condition is termed “N”, and a hypoxic condition is termed “H” accompanied with the value of cg. The conditions with and without flow exposure are noted with the subscripts “flow” and “stat”, respectively. The hypoxic static conditions for the reference in validation experiment are termed “R”.
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