Figure 5
Confirmation of transgene (GaZnF) through southern and Western blot analysis. (a,b) Lane M. 1 kb DNA Ladder, Lane 1. Negative Control (DNA of untransformed plant), Lane 5 is positive control (plasmid DNA of pCAMBIA-1301 with GaZnF gene), Lane 2, 3, 4 & 6, 7 (PF0027, PF0035, PF0039, PF0048 and PF0054 respectively). Selected Transgenic Cotton Plant Samples (GaZnF) were digested with Nco I and Bgl II probed with biotin-labeled GaZnF DNA to assess copy number of the transgene in the transformed plants. The presence of bands at size of positive control confirms the transgene’s integration in transgenic plants as compare to un-transformed plants that is used as negative control. Lane M contains 1 Kb ladder, lane 1 represent negative controls, lane 2, 4 and 6, 7 indicate the integration of GaZnF gene in selected transgenic cotton plant samples, (c) Lane –ve. total soluble protein of the non-transformed cotton plant, Lane + ve, purified GaZnF protein; Lanes 1–4, total soluble proteins of the transgenic cotton plants (PF0035, PF0039, PF0048 and PF0054).
