Figure 6
Quantitative real time PCR to determine the expression of transgenic cotton plants (GaZnF). mRNA samples of Line PF0054 exhibit the different expression levels at both vegetative and flower stages in leaf and stem tissues of transgenic and control plants, GAPDH was used as an internal control for normalization. The means ± SD of three biological replicates are presented. Error bars represent the standard deviation of the mean. Above the bars **** represent significant expression level of transcript in transgenic plants as compare to non- transgenic plants at the P < 0.05 level according to LSD multiple range test.
