Figure 2

The SMG and SLG phenotypes in p130Cas^Δepi– mice. (A–Dʹ) Representative images for immunohistochemical staining of SMG (A–Bʹ) and SLG (C–Dʹ) from P42 p130Cas^flox/flox and p130Cas^Δepi– mice using an anti-p130Cas antibody. Black boxed regions are shown as magnified images. Scale bars = 50 µm (A, B, C, D) and 20 µm (Aʹ, Bʹ, Cʹ, Dʹ). Arrows indicate the GCT in SMG. Arrowheads indicate ducts in SLG. (E) The gross appearance of the male SMG and SLG in P42 p130Cas^flox/flox and p130Cas^Δepi– mice. (F) The total weight of SMG and SLG (salivary gland; SG) from P42 p130Cas^flox/flox and p130Cas^Δepi– mice was measured (p130Cas^flox/flox n = 10 mice, p130Cas^Δepi– n = 8 mice). (G) P42 male p130Cas^flox/flox and p130Cas^Δepi– mice were injected intraperitoneally with EdU. Six hours later, mice were sacrificed and the SMG were extracted and processed for tissue sectioning. EdU positive cells were detected using the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit and nuclei were counterstained using Hoechst 33,342. Arrowheads indicate EdU-positive cells. EdU signals, Hoechst 33,342 and DIC (Differential interference contrast) merged images are shown. The number of Edu-positive cells per section was quantified. Scale bars = 100 µm. (H) Cellular apoptosis was analyzed via the TUNEL assay and nuclei were counterstained with Hoechst 33,342. TUNEL signals, Hoechst 33,342 and DIC merged images are shown. The number of TUNEL-positive cells per field of view was quantified. Scale bars = 25 µm. Data show the means ± SEM, *P < 0.05, **P < 0.01 versus the corresponding p130Cas^flox/flox value.