Figure 2

Landscape of PBMCs (peripheral blood mononuclear cells) from HC (healthy control) and AIDP (acute inflammatory demyelinating polyneuropathy) patients. (a) A 2D UMAP (uniform manifold approximation and projection) visualization of the identified HC and AIDP patients PBMCs clusters. (b) The distribution of HC and AIDP patients derived cells in UMAP, the color of cells are divided by group (C: HC, T: AIDP patients). (c) Cell annotation of HC and AIDP patients PBMCs and visualization by UMAP. (d) A dot plot shows the expression of reference marker genes in annotated celltypes, the plot color scale represents the average expression and the size scale represents the expression percent of selected marker gene transcripts in each cell type. (e) Distribution of classic PBMCs marker genes in each cell of all clusters, the color scale represents expression levels. (f) A violin plot is used to display the expression levels of representative PBMCs marker genes across clusters. (g) Boxplots show proportions of each cell type in HC and AIDP patients. The x axis correspond to the disease course of each patient. n = 3, n = 3 and n = 2 biologically independent samples for HC, AIDP peak stage and AIDP late stage, respectively. Wilcoxon rank-sum test is used to calculate two-sided P values between HC and AIDP peak stage group, shown all P values. Horizontal lines represent median values, with whiskers extending to the farthest data point within a maximum of 1.5 × interquartile range.