Figure 2
Validation of hit compounds from high throughput screens. (a) Dose-dependent effect of selected hit compounds on cTnC-cTnISP interactions monitored by fluorescence polarization. (b) Dose-dependent effect of hit compounds from the cTnC-cTnISP HTS on the ATPase activity of isolated bovine myofibrils at submaximal calcium activation (~ 30% of maximum activation). (c) Dose–response curves for selected hit compounds from the cTnC-cTnIAR HTS. (d) Dose-dependent effect of hit compounds from the cTnC-cTnIAR HTS on the ATPase activity of isolated bovine myofibrils at submaximal calcium activation (~ 70% of maximal activation). (e) Chemical structures of NS5806, Furamidine and Claramine. Means ± SEM, n = 3–7. Statistical significance of values vs control were assessed with a one-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001.
