Figure 5 | Scientific Reports

Figure 5

From: Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays

Figure 5

Biophysical characterization of Claramine-cTnC interaction. (a) ITC for titration of Claramine into cTnC. Insert: Measured steady-state dissociation constants. (b) Left: Part of 2D-1H-15N HSQC spectrum of cTnC in the absence (blue) and in the presence (purple) of Claramine. Right: Plot of chemical shift perturbations (∆δ) of cTnC after addition of excess Furamidine. Chemical shift perturbations mapped onto cTnC structure. (c) ITC titration of unmodified cTnIAR into cTnC in the presence of Claramine (middle). Please note that Claramine had no effect on the cTnC-cTnIAR interaction.

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