Figure 5

ELISA were performed with whole-cell lysate from C. perfringens MLG_7820 and P561, P509, P153, P264 and P537 antibodies raised in immunized birds. Quil-A response is also included. Immuno plate MaxiSorp wells were coated with 0.5 µg of whole-cell lysate from C. perfringens MLG_7820. The error bars representing standard error. The asterisks refer to the level of significance: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, determined by the Mann–Whitney test.