Table 3 Selected candidate proteins with their respective primer sequences and amplicon size after PCR amplification.
Name of candidate | Primer sequence (5’–3’) | Amplicon size (bp) |
|---|---|---|
Hypothetical protein | Forward CACCTTGTTAAATATAGATAGA Reverse TTATTTATTCTTACAACAACCA | 264–2 |
Hypothetical protein | Forward CACCATGAACAAGAACATGGAA Reverse TTATTTATCAAATAATCCTCCA | 153 |
Prepilin N-terminal cleavage methylation domain | Forward CACCGATGTAGCAAGTGCTAAA Reverse TATTAATGACTTGCTGGTGTTG | 509 |
Prepilin N-terminal cleavage methylation domain | Forward CACCAATTATGTAACTTTAGAT Reverse TTATTTTTTATTTCTAAAAGTT | 561 |
Rubrerythrin (low antigenic negative control) | Forward CACCATGAAAAGTTTAAAAGGA Reverse CTAACATTCATTTAAAAGTCTA | 537 |
Sensor Histidine Kinase | Forward CACCTTGGCTATAAAATCGAAA Reverse TTACTTTTTCTTAAAGCTTATT | 2232 |
Formate/nitrite transporter family protein | Forward CACCATGCAAAAAAAAGATATA Reverse TTATTCCATGCTCATAAATTTA | 759 |
FAD-dependent oxidoreductase | Forward CACCATGAGAGAATATGATTTA Reverse TTAACTTATAGTTTTAATAAGC | 891 |
Preprotein translocase YajC | Forward CACCATGAAAACTTTTATGATT Reverse TTATAAAACCTTAGAGACACAA | 264–1 |
SPOR domain-containing protein | Forward CACCGTGAAGTACACTAGAATT Reverse TTATTTGTTTACTTTAAAATTA | 804 |
Isopeptide-forming domain-containing fimbrial protein | Forward CACCATGAAAAATAATATATTA Reverse TTAGTTCTTAGTTTTTCTGCTA | 1569 |
SdpI family protein | Forward CACCATGGGATTTTGGATTTTT Reverse CTACACATTTTTTCTATTGCCA | 384 |
Cna B-type domain-containing protein | Forward CACCATGAAAATAAATAAAAAA Reverse TCATCTTTTCAGTCCTTTATTC | 2091 |
Pilus backbone structural protein | Forward CACCATGATAAACAAGAAAAAA Reverse TTATCTTATAGTTTTACGTCTT | 1074 |
