Figure 4

A high dose of iodide promotes activated B cell death via non-apoptotic cell death. Splenic B cells collected from C57BL/6 J mice were cultured with anti-CD40 antibody, IL-4, and serially diluted KI for three days. (A) The relative survival rate of B cells (compared to that of untreated cells). The data were pooled from two independent experiments and are shown as mean ± SEM (n = 6 for each point). (B) Median fluorescence intensity of 7-AAD. Representative data from two independent experiments are shown as the mean ± SD (n = 3 for each point). (C) Annexin V⁺7-AAD^- early apoptotic B cells (left) and annexin V⁺7-AAD⁺ late apoptotic B cells (right). Representative data from two independent experiments are shown as the mean ± SD (n = 3 for each point). One-way ANOVA with Tukey’s multiple comparison test. n.s.: not significant, *p < 0.05, **p < 0.01. IL, interleukim; KI, potassium iodide.