Figure 5

KI promotes ferroptosis of activated B cells. Splenic B cells collected from C57BL/6 J mice were cultured with anti-CD40 antibody, IL-4, and serially diluted KI for three days. (A) Histogram of 7-ADD expression in B cells stimulated with DMSO or Fer-1. (B,C) Survival rate (B) and MFI of 7-AAD (C) in stimulated B cells with DMSO or Fer-1. Representative data from two independent experiments are shown as the mean ± SD (n = 3 for each point). (D) MFI of ROS in the ROS⁺B220⁺CD45⁺B cells. Representative data from two independent experiments are shown as the mean ± SD (n = 3 for each point). (E) Intracellular ferrous iron measurements in FerroFarRed⁺ cells cultured with or without anti-CD40 and IL-4 for 3 days. (F–I) Quantitative polymerase chain reaction analysis of the relative expression levels of Steap3 (F), Slc11a2 (G), Fth1, and Ftl1 (H) in anti-CD40-stimulated B cells. Data were pooled from two independent experiments and are shown as mean ± SEM (n = 6 for each point). Welch’s t-test. n.s.: not significant, *p < 0.05, **p < 0.01. IL, interleukin; KI, potassium iodide; MFI, mean fluorescence intensity; ROS, reactive oxygen species.