Figure 4

AsA Immunochip-based pathways are supported by summary GWAS from AsA SLE patients. Using SNP-predicted genes from the AsA GWAS validation SNP-set (A) or an equivalently sized cohort of random genes (B) metastructures were generated based on PPI networks, clustered using MCODE and visualized in Cytoscape. Cluster size indicates the number of genes per cluster, edge weight indicates the number of inter-cluster connections and color indicates the number of intra-cluster connections. Enrichment for each cluster was determined by BIG-C and IPA; clusters were then grouped and categorized according to overall function (immune, tissue repair, metabolic, motility or general). Grey boxes indicate categories lacking relevant clusters. (C) Quantitation of cluster size, intra-cluster connections and inter-cluster connections network is displayed. Error bars represent the 95% confidence interval; asterisks (***) indicate a p-value < 0.001 using Welch’s t-test. (D) Quantitation of AsA GWAS (black bars) and random (red) genes falling into each BIG-C category and grouped by overall functionality.