Figure 2

Confocal immunofluorescence imaging of c-Kit and Rho in PN4 and PN8 NRL-GFP+ retina. (a–f) Immunostaining for c-Kit at PN4 (a) and PN8 (d). GFP expression at PN4 (b) and PN8 (e). Merged images at PN4 (c) and PN8 (f). Insets in panels (a) and (b) show a magnification of GFP+ cell labelling positive for c-Kit. (g–l) Rho immunostaining at PN4 (g) and PN8 (j). GFP signal at PN4 (h) and PN8 (k). Merged images at PN4 (i) and PN8 (l). The calibration bar corresponds to 37 µm.