Figure 1

TRPC3 ablation leads to decreased saliva, but increased secretion of Ca²⁺. (A) RT-PCR and immunoblotting for TRPC3 and CaSR in WT and TRPC3-/- (KO) mice SMG ductal cells were performed with GAPDH and β-actin utilized as internal controls, respectively. Each RT-PCR and immunoblotting experiment was performed from n = 3 mice. WT and KO mice (n = 10) saliva samples were collected after stimulation with pilocarpine. (B, C) Volume (total and 0 – 20 min), and (D) flow rate (5 – 20 min) were measured and analyzed for WT and KO mice. (E) Salivary Ca²⁺, (F) Na⁺, (G) K⁺, (H) Cl⁻ measurements were also obtained from (n = 10) WT and KO mice. Full length blots (A) are indicated in the Supplementary Materials. (C, E–H) Bar diagrams are in mean + SEM. *P < 0.05; **P < 0.01.