Figure 2

TRPC3 ablation decreases Ca²⁺ entry. Mean ratiometric (340/380 nm) fluorescence [Ca²⁺]_i traces were performed on (A) WT and (B) TRPC3^−/− (KO) SMG cells. Prior to experiment, Pyr6 (3 μM) or Pyr10 (3 μM) inhibitor was applied to the cells for 10 min, and the [Ca²⁺]_o in the SES buffer was adjusted to 0.5 mM. At start of experiment, for WT cells, L-phenylalanine (L-Phe; 10 mM) was implemented for the Ca²⁺ release at time point ~ 24 s, and then the external solution was adjusted to 2.0 mM Ca²⁺ for the Ca²⁺ entry at time point ~ 155 s for WT cells. For KO cells, L-Phe (10 mM) was implemented for the Ca²⁺ release at time point ~ 11 s, and then the external solution was adjusted to 2.0 mM Ca²⁺ for the Ca²⁺ entry at time point ~ 68 s. Percentage Ca²⁺ entry inhibition comparison of Pyr6 and Pyr10 were performed for (A) WT and (B) KO mice SMG cells. (C-E) WT and (F–H) KO SMG cell I-V curves of L-Phe-activated currents with Pyr6 or Pyr10 inhibition, as well as percentage current inhibition by Pyr6 or Pyr10 (E, H). Bar diagrams are in mean + SEM. *P < 0.05; **P < 0.01.