Figure 4

TRPC3-null mice exhibit greater calcification in SMG. Densitometric analysis of (A) RT-PCR and (B) protein blots for calcification markers (BMP-2, BMP-6, OCL2, OPN4, and RUNX2) in WT and TRPC3 KO (KO) mice SMG tissues were performed using ImageJ. GAPDH and β-actin were utilized as internal control for RT-PCR and immunoblotting, respectively. Each RT-PCR and immunoblotting experiments were performed from n = 3 mice. (C) WT and KO SMG tissue sections were stained with Alizarin Red (AR; pH = 4.3) to detect calcium crystals. Full length blots are indicated in the Supplementary Materials. (A–C) Bar diagrams are in mean + SEM. *P < 0.05; **P < 0.01.