Figure 1

Establishment of resistant cell lines. (A) Ki562 cells were obtained by culturing K562 cells in the presence of increasing doses of imatinib. In parallel, K562 cells were grown without drug selection pressure, in order to accumulate the same level of culture aging. The latter was named Ko562. (B) U251 and LN229 were cultured with increasing doses of TMZ, obtaining U251-R and LN229-R cells, respectively. Both cell lines were also grown without TMZ pressure obtaining U251-V and LN229-V as aging controls.