Figure 3
From: Rational design of phase separating peptides based on phase separating protein sequence of p53
Diffusion dynamics of designed peptide and p53 in their droplets. (A) Schematic diagram of single-molecule tracking of fluorescent peptides or p53 in a droplet. HILO illumination minimizes background fluorescence, enabling single-molecule detection of labeled peptides (green) in droplets. (B) Typical trajectories of Alexa488-labeled designed peptides in corresponding droplets in 45 mM NaCl and 150 mg/mL dextran at pH 7.0. The typical trajectories of single molecules (red) are overlaid in fluorescent images derived based on time averaging (white droplets). (C) Mean square displacement plots of Alexa488-labeled designed peptides or Atto488-labeled p53 in corresponding droplets. The straight lines show the best-fit linear functions for the MSD data. Error bars denote standard errors. (D) Comparison of diffusion coefficients of the designed peptides and p53 in corresponding droplets.
