Figure 6

Tamoxifen-induced CreERT2 activation in pups results in an increase in CD41 + megakaryocytes in the bone marrow. Tamoxifen was injected IP at 75 mg/kg for 3 consecutive days from P9 to P11 and animals were euthanized at P19. (a) Representative images of bone marrow sections of WT (red circle) and R26CreERT2 (blue inverted triangle) pups. CD41 + megakaryocytes are stained in green, CD31 + vessels in red and nuclei in blue. (b) Quantitative analysis of the number of CD41 + megakaryocytes per square millimeter. Data are expressed as mean ± SEM of n = 5–8 mice per group. Mann–Whitney tests were used to assess statistical significance (**p-value ≤ 0.01). scale bars 100 µm.