Figure 1
From: Development and characterization of agonistic antibodies targeting the Ig-like 1 domain of MuSK
Ig-like 1 binding MuSK antibodies have varying agonist potential in vitro. (a) Graphic depiction of the epitopes of the Ig-like 1 agonist antibodies on MuSK. Antibodies in the same box share an epitope. Overlapping boxes represent partial sharing of an epitope. Bold-underscored clones represent the antibodies produced in llamas. (b) MuSK phosphorylation levels induced by MuSK agonist antibodies as expressed by percentage normalized to agrin only treatment. Colors identify groups of antibody clones derived from the same parental clone. (c) Percentage of AChR clusters induced by MuSK agonist antibodies, with a size larger than 15 µm2, normalized to agrin only treatment. (d) Percentage of different AChR cluster sizes induced by the different candidate antibodies normalized to the agrin only condition. Data information: for statistical analysis in b and c, an unpaired t-test with Bonferroni correction was performed to compare the different agonists versus biotin control. Statistical significance was set at *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
