Figure 2

MuSK agonist antibodies (partially) rescue AChR declustering induced by monovalent monoclonal MuSK antibodies or polyclonal patient IgG. (a) Outline of experimental set up. (b–d) Percentage of AChR clustering with a size larger than 15 µm² induced by an agonist antibody or negative control antibody (Mota) with (+) or without (−) 13-3B5/b12 antibody (b), 11-3F6/b12 antibody (c) or MuSK MG polyclonal IgG (d) together with agrin. Data information: all data is normalized to the agrin only condition. Percentages of rescue are calculated with the following formula, taking the levels of negative controls in account: \(\frac{\left(agrin+inhibition+agonist\right)-\left(agrin+inhibition\right)}{agrin only-untreated}\times {100}\). For statistical analysis, an unpaired t-test with Bonferroni correction was performed to compare the different agonists versus Mota control. Statistical significance was set at *P < 0.05; **P < 0.01; ***P < 0.001. #mAb13 experiments were performed with separate untreated and Mota conditions (not displayed), the 8 datapoints of mAb13 with and without agrin + Pt Total IgG were gathered from repetitions within two experiments instead of four individual experiments.