Figure 2 | Scientific Reports

Figure 2

From: The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos

Figure 2

The optimal time for pre-IVM incubation of porcine oocytes with rolipram–cilostamide treatments. Percentage of oocytes with open, partially open, and closed GJC after 2 (A), 4 (B), 6 (C), and 8 (D) h of pre-IVM incubation. A significant difference (P < 0.05) was observed between the control and rolipram–cilostamide-treated groups for open, partially open, and closed GJC at 2 and 4 h but not 6 or 8 h. The percentage of oocytes with open and partially open GJC was less than 10%. As seen in (A,B), after 4 h of pre-IVM incubation, rolipram–cilostamide treatment significantly affected functional GJC status, and this period was determined to be the optimal time for the two-phase IVM of porcine oocytes using rolipram and cilostamide.

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