Figure 6
Effect of combined two-phase IVM with rolipram and cilostamide on the embryonic development of transgenic cloned porcine embryos. Representative images of blastocysts developed from oocytes matured in control (A) and rolipram–cilostamide (B) during IVM. At least 68 blastocysts were stained from each group. (C) Inner cell mass to TE ratio in differential staining. The inner cell mass to TE ratio in blastocysts from two-phase IVM differed significantly from that from the control (0.399 vs 0.269, respectively, P < 0.05). The nuclei of ICM and TE cells were stained with Hoechst (blue) and PI (red) dye, respectively. The data are from at least three independent experiments, and the values represent the mean ± SEM.
