Table 1 Primer sequences.
From: Drug-induced loss of imprinting revealed using bioluminescent reporters of Cdkn1c
Application/target | Forward | Reverse |
|---|---|---|
Genotyping | ||
 Cdkn1c-Fluc-lacZ55 | CTCCATGCGATCACAGTGG | CTTTGGATCCAGTGGACTGG |
 Sex95 | CTGAAGCTTTTGGCTTTGAG | CCACTGCCAAATTCTTTGG |
Bisulphite sequencing | ||
 KvDMR41 | TAAGGTGAGTGGTTTAGGAT | AATCCCCCACACCTAAATTC |
 sDMR55 | AGTATAATGTAGTATTTTTAGT | AAAACTATACCCAACTCCATA |
RT-qPCR | ||
 β-Actin | CATCCGTAAAGACCTCTATGCCAAC | ATGGAGCCACCGATCCACA |
 WT Cdkn1c29 | AGAGAACTGCGCAGGAGAAC | TCTGGCCGTTAGCCTCTAAA |
 Cdkn1c-Fluc-lacZ55 | AGAGAACTGCGCAGGAGAAC | GTTCCATCTTCCAGCGGATA |
 Total Cdkn1c | TCCTCCCGTGGCATTAAAGG | CTTAGCTGCACCCCTACCAG |
 Kcnq1ot147 | TTGGATTACTTCGGTGGGCT | ACACGGATGAAAACCACGCT |
 Oct4 | AGAGGATCACCTTGGGGTACA | CGAAGCGACAGATGGTGGTC |
