Figure 4 | Scientific Reports

Figure 4

From: Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats

Figure 4

Within 10 min after the phosphate intervention, FEPi and phosphaturia increased in both the PV and CV groups. (a) Experimental design of (b) to (d). The normal rats received phosphate interventions in the same manner as shown in Fig. 2a. The rats at 5 min after the phosphate interventions were evaluated in Fig. 2c,d, while rats at 10 min were evaluated in Fig. 4. (b) ΔFEPi between 0 and 10 min and urinary phosphate excretion during the 10-min period (n = 5 rats per group). (c) Representative immunofluorescence images of renal PTECs stained with phalloidin (green) and NaPi-2a (red) at 10 min. The upper and the lower images in each group are the immunofluorescence images with higher and lower magnifications, respectively (scale bars: 5 µm or 20 µm). (d) Plasma intact-parathyroid hormone (i-PTH), intact-fibroblast growth factor 23 (i-FGF23), and serum 1,25-dihydroxyvitamin D (1,25VD) concentrations at 0 and 10 min (n = 4–5 rats per group). Plasma and serum samples were collected from the inferior vena cava. The results are presented as the means ± SDs, n.s.: not significant, Ref.: reference. *P < 0.05 based on Dunnett’s test in (b) and (d).

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