Figure 5 | Scientific Reports

Figure 5

From: Hepatic phosphate uptake and subsequent nerve-mediated phosphaturia are crucial for phosphate homeostasis following portal vein passage of phosphate in rats

Figure 5

Rats in the IVC and PV groups increased FEPi and phosphaturia within the first 10 min in a dopamine D1-like receptor-dependent manner. (a) Experimental design for (b) and (c). Rats pretreated with SCH23390, a D1-like dopamine receptor antagonist, were randomly divided into two groups, IVC-SCH and PV-SCH. Vehicle-pretreated (normal saline) rats were randomly divided into three groups, Ctrl-veh, IVC-veh, and PV-veh. Five minutes after these pretreatments, rats in groups with Ctrl in the group name, rats in groups with IVC in the group name, and rats in groups with PV in the group name were subjected to phosphate interventions similarly to the rats in the Ctrl, IVC, and PV groups, respectively. (b) ΔFEPi between 0 and 10 min and urinary phosphate excretion during the 10-min period (n = 5 rats per group). (c) Representative immunofluorescence images of renal PTECs stained with phalloidin (green) and NaPi-2a (red) at 10 min. The upper and the lower images in each group are the immunofluorescence images with higher and lower magnification, respectively (scale bars: 5 µm or 20 µm). The results are presented as the means ± SDs, n.s.: not significant, Ref.: reference. *P < 0.05 based on Dunnett’s test in (b).

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