Figure 6

Phosphorylation of endothelial nitric oxide synthase (eNOS) and levels of phosphate incorporation to calciprotein particles (CPPs) were suppressed in the PV group. (a) Representative immunoblots of phosphorylated eNOS (Thr495) and total eNOS in the aorta. Aortic tissues were collected at 20 min in the rats shown in Fig. 2a. The original blots are presented in Supplementary Fig. S11. (b) Quantifying phosphorylated eNOS (Thr495) (n = 7 rats per group). (c) Experimental design of (d). Levels of phosphate incorporation to CPPs were analyzed. BNX rats were randomly divided into three groups, Ctrl-BNX, IVC-³²P-BNX, and PV-³²P-BNX. Sixteen hours after the BNX operation, rats in the Ctrl-BNX group were similarly administered normal saline to the rats in Ctrl group. Rats in the IVC-³²P-BNX and PV-³²P-BNX groups were administered a radiolabeled phosphate solution similar to those in IVC-³²P and PV-³²P groups. At 10 min, blood samples were collected from the inferior vena cava. After the separation of serum (1200 × g for 15 min), levels of ³²P in the pellet fraction (16,000 × g for 2 h) obtained from the serum were measured (n = 4 rats per group). All results are presented as the means ± SDs, n.s.: not significant, Ref: reference. *P < 0.05 based on Dunnett’s test in (b) and (d).