Figure 2 | Scientific Reports

Figure 2

From: Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Figure 2

Establishment of MFAP5-knockdown C3H10 and 3T3-L1 cells and the role of MFAP5 in adipogenesis. (A) Western blotting of MFAP5 protein levels in the blank, control, and MFAP5-shRNA1-3 groups of C3H10 and 3T3-L1 cells. (B–C) Quantification of protein levels using ImageJ software in the blank, control, and MFAP5-shRNA1-3 groups of C3H10 and 3T3-L1 cells, respectively. (D–E) qRT-PCR of MFAP5 mRNA levels in the blank, control, and MFAP5 -shRNA1-3 groups of C3H10 and 3T3-L1 cells. (F) Lipid droplets accumulation determined by Oil red O staining. (G) Absorbance at 510 nm of Oil red O-stained cells. (H–J) Expression of CEBPα, FABP4, and SREB1 during adipogenesis was suppressed in MFAP5-knockdown cells. n = 3, the experiment was repeated 3 times. Values are means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.

Back to article page