Figure 4 | Scientific Reports

Figure 4

From: Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ

Figure 4

Silencing of SND1 in MFAP5-knockdown cell lines reversed the promotion of adipogenesis. (A) Western blotting of SND1 in MFAP5-knockdown cells compared to the control. (B) SND1 knockdown reversed the activation of PPARγ downstream proteins, including CD36 and Adipsin, in MFAP5-knockdown cells before and after adipogenic induction. (C) Accumulation of lipid droplets by Oil red O staining 10 days after adipogenic induction in the control, MFAP5-sh, and MFAP5-SND1-sh groups. (D–F) Relative expression levels of adipogenic biomarkers—CEBPα, FABP4, and SREB1—by qRT-PCR on day 10 of adipogenesis. n = 3, the experiment was repeated 3 times. Values are means ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001.

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