Figure 4

Identification of two distinct m6A modification pattern subtypes in ICM. (A) Consensus clustering cumulative distribution function (CDF) for k = 2–9. (B) Relative changes in the area under the CDF curve for k = 2–9. (C) Heatmap of the matrix of the co-occurrence proportions in the ICM samples. (D) Principal component analysis of the transcriptome profiles of the two m6A subtypes, showing a remarkable difference in the transcriptome between the different modification patterns. The box plot (E) and heatmap (F) showing that the expression of seven m6A regulators were significantly different between the m6A-cluster-A and -B groups. *, P < 0.05; ***, P < 0.001.