Table 4 Detailed information and amplification profile of the ISSR primers used in the study.
From: Histological and molecular insights in to in vitro regeneration pattern of Xanthosoma sagittifolium
S. no. | Primer code | Primer sequence (5'–3') | Annealing temperature (°C) | Total no. of bands | Band size range (bp) |
|---|---|---|---|---|---|
IS6 | (GA)8C | 51.2 | 5 | 110–486 | |
IS7 | (GT)8A | 50.7 | NA | NA | |
IS8 | (AG)8C | 51.2 | 5 | 199–515 | |
IS9 | (TG)7 T A | 54.1 | 2 | 262–920 | |
IS10 | C(GA)8 | 49.2 | Smear | Smear | |
IS11 | (CA)7G | 52.5 | 5 | 166–560 | |
IS12 | (GT)8C | 52.5 | NA | NA | |
IS61 | (GA)8 T | 54.1 | 4 | 267–594 | |
IS53 | (AG)7C | 54.1 | 4 | 177–418 | |
IS65 | (AG)8 T | 52.5 | Smear | Smear | |
UBC859 | (TG)8RC | 51.2 | 4 | 239–996 | |
UBC824 | (TC)8G | 50 | 2 | 298–495 | |
UBC861 | A(CCA)8CC | 56.6 | 5 | 250–736 | |
UBC858 | (TG)8RT | 52.5 | 1 | 215 | |
UBC870 | (TGC)6 | 51.2 | Smear | Smear | |
UBC825 | (AC)8 T | 52.5 | 3 | 230–424 | |
UBC826 | (AC)8C | 52.5 | 3 | 192–600 | |
UBC828 | (TG)8A | 50 | Smear | Smear | |
UBC829 | (TG)8C | 50 | 1 | 237 | |
UBC831 | (AT)8YA | 37 | NA | NA | |
UBC832 | (AT)8YC | 37 | NA | NA | |
UBC833 | (AT)8YG | 42.8 | NA | NA | |
UBC834 | (AG)8YT | 52.5 | 1 | 234 | |
UBC835 | (AG)8YC | 52.5 | 5 | 142–722 | |
UBC836 | (AG)8YA | 50 | 4 | 243–688 | |
UBC840 | (GA)8YT | 50 | 5 | 197–573 | |
UBC841 | (GA)8YC | 53.6 | NA | NA | |
UBC842 | (GA)8YG | 54.1 | 4 | 276–499 | |
UBC843 | (CT)8RA | 50 | NA | NA | |
UBC846 | (CA)8AT | 50 | 3 | 175–328 | |
UBC847 | (CA)8AC | 50 | 5 | 194–684 | |
UBC848 | (CA)8AG | 50 | 4 | 164–589 | |
UBC849 | (GT)8CA | 50 | Smear | Smear | |
UBC850 | (GT)8CC | 50 | 4 | 110–590 | |
UBC851 | (GT)8CG | 50 | Smear | Smear | |
UBC852 | (TC)8RA | 50 | Smear | Smear | |
UBC853 | (TC)8AT | 50 | 2 | 203–396 | |
UBC854 | (TC)8AG | 50 | Smear | Smear | |
UBC855 | (AC)8CT | 50 | 7 | 125–750 | |
UBC856 | (AC)8CA | 50 | 3 | 335–650 |
