Figure 1
From: PDLIM1 inhibits cell migration and invasion in diabetic retinopathy via negatively regulating Wnt3a
The expression of PDLIM1 in mouse retina and retinal capillary endothelial cells (RCECs). (A) Immunofluorescence staining shows that PDLIM1 is mainly expressed in the retinal pigment epithelium (RPE). Scale bar: 100 μm. (B) Semi-quantitative analysis showed that PDLIM1 expression decreased in DR mice compared to control. (C) Representative immunoblots show that the protein expression of PDLIM1 decreased after HG/HL treatment. (D) PDLIM1 decreased at 24 h in HG/HL (P < 0.05), and the trend was more evident after 72 h (P < 0.01) compared with those in NG/NL. (E) The mRNA expression of PDLIM1 downregulated at 24 h in HG/HL (P < 0.05), and the trend was more evident after 72 h (P < 0.01) compared with those in NG/NL. *P < 0.05, **P < 0.01. (CON control group, DR diabetic retinopathy, GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer, RPE: retinal pigment epithelium).
