Figure 1
From: Quantification of bulk lipid species in human platelets and their thrombin-induced release
Workflow platelet release assay. Washed platelets were adjusted to a defined cell count (100/nL). One aliquot was stored immediately as basal platelets, a second aliquot was stimulated by thrombin. After a 15 min stimulation and centrifugation, pellet and supernatant were stored as activated platelets and their release, respectively. Samples were analyzed by quantitative lipidomics using flow injection analysis (FIA) coupled with high-resolution mass spectrometry (HRMS) as well as tandem mass spectrometry (MS/MS). The study covered major lipid classes: cholesteryl ester (CE), ceramide (Cer), free cholesterol (FC), hexosylceramide (HexCer), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylcholine (PC), phosphatidylcholine ether (PC O), phosphatidylethanolamine (PE), PE plasmalogens (PE P), phosphatidylinositol (PI), phosphatidylserine (PS), sphingomyelin (SM), trigylcerides (TG).
