Figure 5 | Scientific Reports

Figure 5

From: ALS-linked TDP-43 mutations interfere with the recruitment of RNA recognition motifs to G-quadruplex RNA

Figure 5

GR mutations interfere with the recruitment of RRM. (A) A cartoon of experimental flow. (B) The His-RRM protein (Fig. S5) was co-pulled with GST-GR wild-type and mutant proteins by glutathione sepharose beads (GSB) in the absence and presence of G4-RNAs, PSD-95 and CaMKIIα (Table S2) and detected by western blotting analysis (for original images, see Fig. S6). The graphs show the average of G4 dependent binding ratio compared to the GST-GR wild-type protein and the SEM of three experiments is shown. Two-tailed Student’s t-test was used to determine the statistical significance **P < 0.01.

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