Figure 3
From: In vitro PCR verification that lysozyme inhibits nucleic acid replication and transcription
After coculture of HepG2 cells with lysozyme for 24 h, the cell lysate and supernatant of HepG2 cells showed different effects on the PCR process in vitro. (A)–(F) Gel electrophoresis of PCR products. The supernatant (A)–(C) and cell lysate (D)–(F) of HepG2 cells were added to the reaction system: (A/D) replication, (B/E) transcription and (C/F) reverse transcription. Intensity analysis of target bands in gel electrophoresis results shown to the top of the blot. Data are representative of 2 independent experiments and presented as the mean ± SD; *p < 0.05, ***p < 0.001. Significant differences were evaluated using one-way ANOVA with a post hoc test (Fisher’s least significant difference).
