Figure 4
From: In vitro PCR verification that lysozyme inhibits nucleic acid replication and transcription
Artificial gastric juice and rabbit liver tissue lysate-treated lysozyme blocks EmGfp, Amp and Grp78 DNA replication, transcription and reverse transcription in vitro. (A)–(F) Gel electrophoresis of PCR products. Artificial gastric juice-treated lysozyme (A)–(C) and rabbit liver tissue lysate-treated lysozyme (D)–(F) were added to the reaction system: (A/D) replication, (B/E) transcription and (C/F) reverse transcription. Intensity analysis of target bands in gel electrophoresis is shown at the top of the blot. Data are representative of 2 independent experiments and presented as the mean ± SD; *p < 0.05, ***p < 0.001. Significant differences were evaluated using one-way ANOVA with a post hoc test (Fisher’s least significant difference).
