Figure 5
From: In vitro PCR verification that lysozyme inhibits nucleic acid replication and transcription
Rabbit jejunum lavage fluid-treated lysozyme partially blocks EmGfp, Amp and Grp78 DNA replication, transcription and reverse transcription in vitro. (A)–(F) Gel electrophoresis of PCR products. Lysozyme treated with lavage fluid of the upper (A)–(C) and lower (D)–(F) jejunum was added to the reaction system: (A/D) replication, (B/E) transcription and (C/F) reverse transcription. Intensity analysis of target bands in gel electrophoresis is shown at the top of the blot. Data are representative of 2 independent experiments and presented as the mean ± SD; *p < 0.05, ***p < 0.001. Significant differences were evaluated using one-way ANOVA with a post hoc test (Fisher’s least significant difference).
