Figure 1 | Scientific Reports

Figure 1

From: Assessment of a multisite standardized biospecimen collection protocol for immune phenotyping in neurodevelopmental disorders

Figure 1

Quality control data. (A) Frequency distribution of Trypan Blue counts in thawed samples. The percent frequency of post-thaw sample PBMC counts measured using the Trypan Blue exclusion test. Samples containing more than 500,000 cells, indicated by the dotted line, proceeded to quality control phase two. (B) Frequency distribution of alive CD45+ Cells in thawed samples. The percent frequency of CD45+ cells that were negative for the Zombie Red viability stain as a percent of CD45+ cells in the sample. Samples with a survival rate of 70%, indicated by the dotted line, were considered viable for future use.

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