Figure 3
From: Polypropylene microplastics promote metastatic features in human breast cancer
Effect of PPMP on breast cancer cell motility. (A) Both breast cancer cell lines were incubated with PPMPs and observed under a time-lapse imaging microscope for six hours. The plots depict the motility of individual cells in the representative experiment. (B) Quantification of the migration speed reveals unchanged cellular velocity by the PPMPs treatment in both cell lines. The results are expression as the mean ± SEM; n = 360 individual cells for both cell lines. (unpaired two-tailed t test, p = 0.7629 for the MDA-MB-231 cells and p = 0.1116 for the MCF-7 cells). (C) and (D) Effects of the PPMPs on the ability of wound healing in the MDA-MB-231 and MCF-7 cell lines. Photos in each group were taken at 0 hours and 12 hours after the PPMP treatment. The closed area (indicated yellow line) was quantified by Image J software. Experiments were repeated independently three times for the MCF-7 cells and five times for the MDA-MB-231 cells (unpaired two-tailed t test; p = 0.0684 for the MDA-MB-231 cells, p = 0.7760 for the MCF-7 cells).
