Table 1 Results of crRNA performance testing.

From: Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target

Target

Lineage

Numbera

Performanceb

High

Medium

Low

GPC

II

27

12 (44%)

13 (48%)

2 (7%)

IV

37

13 (35%)

20 (54%)

4 (11%)

L

II

50

7 (14%)

37 (74%)

6 (12%)

IV

50

14 (28%)

27 (54%)

9 (18%)

  1. aThe number of crRNAs tested, bcrRNAs were classified into three groups based on the integrated, background subtracted, fluorescent signal relative to the highest signal obtained. Three performance classes were defined: high performance (with signal at 80% or higher), intermediate (signal lower than 80% but higher than 20%) and low (signal at 20% or lower).
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